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E. coli BL21 Gold LC-MSMS in varying growth conditions

ABSTRACT: Proteomic data from LC-MSMS of E. coli BL21 Gold grown in varying growth conditions, including in LB, M9, and spent mammalian cell media.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Ciara Lynch

LAB HEAD: O'Connell, David

PROVIDER: PXD026884 | Pride | 2022-05-20

REPOSITORIES: Pride

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Conversion of mammalian cell culture media waste to microbial fermentation feed efficiently supports production of recombinant protein by Escherichia coli.

Lynch Ciara D CD O'Connell David J DJ

PloS one 20220504 5

Deriving new value from waste streams through secondary processes is a central aim of the circular bioeconomy. In this study we investigate whether chemically defined spent media (CDSM) waste from cell culture bioprocess can be recycled and used as a feed in secondary microbial fermentation to produce new recombinant protein products. Our results show that CDSM supplemented with 2% glycerol supported a specific growth rate of E. coli cultures equivalent to that achieved using a nutritionally ric ...[more]

PMID: 35507546

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