Dynamic proteomics of mesenchymal stem cells in critical limb ischemia
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Skeletal Muscle, Mesenchymal Cell
DISEASE(S): Limb Ischemia
SUBMITTER: 
Yipeng Du
LAB HEAD: Zhiliang Li
PROVIDER: PXD026954 | Pride | 2021-09-07
REPOSITORIES: Pride
Publications
Deciphering the <i>in vivo</i> Dynamic Proteomics of Mesenchymal Stem Cells in Critical Limb Ischemia.
Du Yipeng Y Li Xiaoting X Yan Wenying W Zeng Zhaohua Z Han Dunzheng D Ouyang Hong H Pan Xiudi X Luo Bihui B Zhou Bohua B Fu Qiang Q Lu Dongfeng D Huang Zheng Z Li Zhiliang Z
Frontiers in cell and developmental biology 20210630
<h4>Objective</h4>Regenerative therapy using mesenchymal stem cells (MSC) is a promising therapeutic method for critical limb ischemia (CLI). To understand how the cells are involved in the regenerative process of limb ischemia locally, we proposed a metabolic protein labeling method to label cell proteomes <i>in situ</i> and then decipher the proteome dynamics of MSCs in ischemic hind limb.<h4>Methods and results</h4>In this study, we overexpressed mutant methionyl-tRNA synthetase (MetRS), whic ...[more]
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