Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis

ABSTRACT: Label-free quantitative proteomics was employed to compare the protein content of extracellular vesicles isolated by various differential centrifugation-based approaches from expressed prostatic secretions in urine (EPS-urine) from men with prostate cancer. The developed optimized approach improved EV purity by depleting the high-abundance urine protein Tamm-Horsfall protein (THP) and other common contaminants and achieved relative enrichment of prostate cancer-associated EV-resident proteins.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

DISEASE(S): Prostate Cancer

SUBMITTER: Vanessa Correll  

LAB HEAD: O. John Semmes

PROVIDER: PXD026974 | Pride | 2022-02-09

REPOSITORIES: Pride