Dataset Information

Filariasis test strip (FTS) antibody recognition of Brugia malayi glycoproteins

ABSTRACT: Rapid and accurate prevalence mapping of lymphatic filariasis (LF) is necessary to eliminate this disfiguring and disabling neglected tropical disease. Unfortunately, rapid tests such as the filariasis test strip (FTS) for Wuchereria bancrofti, the causative agent of LF in Africa, can cross-react with antigens circulating in some persons infected by the African eye worm, Loa loa, rendering the test unreliable in eleven co-endemic nations. The intended target of the FTS is a heavily glycosylated W. bancrofti circulating filarial antigen (Wb-CFA). Previously, we determined that the FTS monoclonal antibody, AD12.1, which detects a carbohydrate epitope on Wb-CFA, also detects multiple L. loa proteins in cross-reactive sera from persons with loiasis. Since the carbohydrate epitope recognized by AD12.1 is present on glycoproteins of other parasitic nematodes, including Brugia species, it is unclear why reactive glycoproteins are not detected in infections with other filarial parasites. To gain a better understanding of the proteins recognized by the FTS diagnostic antibody, we used proteomics and to characterize filarial glycoproteins that are bound by the AD12.1 antibody using Brugia malayi as a model. This included using shotgun proteomics to catalog the AD12-reactive glycoproteins found in adult female somatic (BmSOM) and excretory/secretory (BmES) antigens. As well as in-gel proteomics of two predominant protein bands from BmES.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Brugia Malayi

DISEASE(S): Filarial Elephantiasis

SUBMITTER: Marla Hertz

LAB HEAD: Philip J Budge

PROVIDER: PXD027003 | Pride | 2023-09-28

REPOSITORIES: Pride

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Publications

Identification and characterization of Loa loa antigens responsible for cross-reactivity with rapid diagnostic tests for lymphatic filariasis.

Hertz Marla I MI Nana-Djeunga Hugues H Kamgno Joseph J Jelil Njouendou Abdel A Chawa Chunda Valerine V Wanji Samuel S Rush Amy A Fischer Peter U PU Weil Gary J GJ Budge Philip J PJ

PLoS neglected tropical diseases 20181116 11

The Global Program to Eliminate Lymphatic Filariasis (LF) relies on rapid diagnostic tests (RDTs) to determine where annual mass drug administration for LF is required and when it can be stopped. These tests detect a Wuchereria bancrofti glycoprotein in the blood of infected persons via a carbohydrate moiety recognized by the monoclonal antibodies AD12 and DH6.5. Loiasis cross-reactivity with LF RDTs has recently been recognized as a serious obstacle to LF elimination in loiasis-endemic areas. T ...[more]

PMID: 30444866

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Dataset Information

Filariasis test strip (FTS) antibody recognition of Brugia malayi glycoproteins

Publications

Identification and characterization of Loa loa antigens responsible for cross-reactivity with rapid diagnostic tests for lymphatic filariasis.

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