Determination of carry-over contaminations in recombinant major peanut allergen Ara h 2 produced in Nicotiana benthamiana leaves and analysis of proline hydroxylation patterns

ABSTRACT: The major peanut allergen Ara h 2 undergoes site-specific hydroxylation at three proline residues within repetitive IgE-binding DPYSPOHS sequences. There has been no report of recombinant Ara h 2 production with hydroxyprolines until now. We produced recombinant Ara h 2 in N. benthamiana leaves to determine host-related contaminants and to analyze site-specific hydroxylation of proline residues.

INSTRUMENT(S):

ORGANISM(S): Arachis Hypogaea Var. Vulgaris

SUBMITTER: Dorothea Anrather  

LAB HEAD: Heimo Breiteneder

PROVIDER: PXD027015 | Pride | 2021-09-28

REPOSITORIES: Pride