Proteomics

Dataset Information

0

Proteomic analysis of the pathogen surface in cholera identifies host proteins involved in defense and microbiota recognition


ABSTRACT: The microbial cell surface is a critical site of microbe-host interactions that often control infection outcomes. Defining the set of host proteins present at this interface has been challenging. Here, we used a surface biotinylation approach coupled to quantitative mass spectrometry to identify and quantify both bacterial and host proteins present on the surface of diarrheal fluid-derived V. cholerae in an infant rabbit model of cholera. Our data showed that V. cholerae surfaces were coated with numerous host proteins, whose abundance were driven by the presence of cholera toxin, including the C type lectin SP-D. Mice lacking SP-D had enhanced V. cholerae intestinal colonization. Additional host proteins (AnxA1, LPO and ZAG) capable of binding V. cholerae were also found to recognize distinct taxa of the murine intestinal microbiota, suggesting that these host factors may play roles in intestinal homeostasis in addition to host defense. Proteomic analysis of microbial surfaces is valuable for identifying host interactions that regulate infection and homeostasis with both pathogens and endogenous microbiota alike.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Vibrio Cholerae Serotype O1 (strain Atcc 39315 / El Tor Inaba N16961) Oryctolagus Cuniculus (rabbit)

SUBMITTER: Abdelrahim Zoued  

LAB HEAD: Matthew K Waldor

PROVIDER: PXD027076 | Pride | 2021-10-02

REPOSITORIES: Pride

Similar Datasets

2021-07-08 | GSE179529 | GEO
2021-07-08 | GSE179528 | GEO
2016-02-22 | PXD003000 | Pride
2015-10-26 | E-GEOD-73974 | biostudies-arrayexpress
2005-08-17 | GSE2775 | GEO
2008-06-11 | E-GEOD-10314 | biostudies-arrayexpress
2020-09-28 | PXD017801 | Pride
2010-06-09 | E-GEOD-2775 | biostudies-arrayexpress
2022-12-01 | GSE213598 | GEO
2013-08-13 | E-GEOD-38272 | biostudies-arrayexpress