Novel recognition site for polyUb and UBL signals in Rpn1

ABSTRACT: Rpn1 is the largest proteasomal receptor for polyubiquitin and shuttle proteins contains one known binding site for polyubiquitin and shuttle proteins. We used a combination of NMR spectroscopy, photo-crosslinking, mass spectrometry, and mutagenesis,to confirm the presence of additional ubiquitin recognition site on RPN1. This novel site is situated in the N-terminal section of Rpn1, a region previously surmised to be devoid of functionality.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Oded Kleifeld  

LAB HEAD: David Fushman

PROVIDER: PXD027128 | Pride | 2021-08-09

REPOSITORIES: Pride