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Kinome profiling of primary CLL cells

ABSTRACT: Investigation of BCR signalling response of primary cells from a cohort of 40 CLL patients using kinobbead idolation and MS profiling. Cells were either stimulated using anti-IgM or isotype control.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Blood

SUBMITTER: Adam Linley

LAB HEAD: Adam Linley

PROVIDER: PXD027131 | Pride | 2023-03-11

REPOSITORIES: Pride

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Publications

Kinobead Profiling Reveals Reprogramming of BCR Signaling in Response to Therapy within Primary CLL Cells.

Linley Adam J AJ Karydis Laura I LI Mondru Anil K AK D'Avola Annalisa A Al Shmrany Humood H Cicconi Silvia S Griffin Rebecca R Forconi Francesco F Pettitt Andrew R AR Kalakonda Nagesh N Rawstron Andrew C AC Hillmen Peter P Steele Andrew J AJ MacEwan David J DJ Packham Graham G Prior Ian A IA Slupsky Joseph R JR

Clinical cancer research : an official journal of the American Association for Cancer Research 20210811 20

<h4>Purpose</h4>B-cell receptor (BCR) signaling is critical for the pathogenesis of chronic lymphocytic leukemia (CLL), promoting both malignant cell survival and disease progression. Although vital, understanding of the wider signaling network associated with malignant BCR stimulation is poor. This is relevant with respect to potential changes in response to therapy, particularly involving kinase inhibitors. In the current study, we describe a novel high-resolution approach to investigate BCR s ...[more]

PMID: 34380642

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