Search for the interactors of Arabidopsis thaliana myosin receptor MRF7

ABSTRACT: This project aims at identifying the binding partners of the Arabidopsis thaliana MRF7 protein and of its truncated form ΔMRF7, which lacks a potential protein binding domain DUF593 at the N-terminus. A.thaliana plants stably overexpressing GFP-MRF7 and GFP-ΔMRF7 were generated by floral dipping of Col-0 plants. Co-immunoprecipitation was carried out using the GFP-Trap®_A beads technology (Chromotek) and samples thus obtained were analysed through Liquid Chromatography-Mass Spectrometry

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Root, Shoot

SUBMITTER: Chiara Perico  

LAB HEAD: Imogen Sparkes

PROVIDER: PXD027155 | Pride | 2022-02-17

REPOSITORIES: Pride