The inflammation repressor TNIP1 is degraded by selective autophagy in a LIR-dependent manner upon TLR3 activation

ABSTRACT: In the project « The inflammation repressor TNIP1 is degraded by selective autophagy in a LIR-dependent manner upon TLR3 activation » Jianwen Zhou, Nikoline Lander Rasmussen, Vyacheslav Akimov, Blagoy Blagoev, Trond Lamark, Terje Johansen and Jörn Dengjel performed MS-based proteomics to study the function of TNIP1. Three set of SILAC experiments were performed (classical double-triple labeling): (1) Global analysis of ubiquitination events using the UbiSite approach. Three biological replicates were performed using following SILAC labeling (1) Protein-protein interactions of TNIP1 performing HA-TNIP1 AP-MS analyses. Three biological replicates were performed using following SILAC labeling (3) Expression proteomics comparing WT and different TNIP1 ko clones. Six biological replicates were performed using following SILAC labeling

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Joern Dengjel  

LAB HEAD: Joern Dengjel

PROVIDER: PXD027163 | Pride | 2022-08-07

REPOSITORIES: Pride