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Acinetobacter baumannii Ci79 WT and TrxA mutant outer membrane vesicle proteins

ABSTRACT: To investigate whether OMVs can be modulated by TrxA, we isolated OMVs from wild type (WT) and TrxA-deficient (ΔtrxA) A. baumannii clinical isolate Ci79, and carried out a functional and proteomic comparison.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Acinetobacter Baumannii Ci79

TISSUE(S): Vesicle Budding From Membrane

DISEASE(S): Disease Free

SUBMITTER: Jieh-Juen Yu

LAB HEAD: Bernard Arulanandam

PROVIDER: PXD027181 | Pride | 2022-08-12

REPOSITORIES: Pride

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Thioredoxin-mediated alteration of protein content and cytotoxicity of Acinetobacter baumannii outer membrane vesicles.

Shrihari Swathi S May Holly C HC Yu Jieh-Juen JJ Papp Sara B SB Chambers James P JP Guentzel M Neal MN Arulanandam Bernard P BP

Experimental biology and medicine (Maywood, N.J.) 20211029 3

Acinetobacter baumannii is a Gram-negative bacterium responsible for many hospital-acquired infections including ventilator-associated pneumonia and sepsis. We have previously identified A. baumannii thioredoxin A protein (TrxA) as a virulence factor with a multitude of functions including reduction of protein disulfides. TrxA plays an important role in resistance to oxidative stress facilitating host immune evasion in part by alteration of type IV pili and cell surface hydrophobic ...[more]

PMID: 34713732

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