Proteomics

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Phosphorylation of extracellular proteins in Acinetobacter baumannii in sessile mode of growth

ABSTRACT: Here, we have investigated, for the first time, the serine, threonine and tyrosine phosphoproteome in the extracellular medium of A. baumannii during biofilm mode of growth for two strains: the reference strain ATCC 17978 and the virulent multi-drug resistant strain AB0057, a clinical isolate from a bloodstream infection. Overall, a total of 109 and 146 phosphorylated proteins were identified for ATCC 17978 and AB0057, respectively. We also showed that one specific phosphorylation site was essential for the activity of the A. baumannii type VI secretion system. This provides the first global phosphosecretome profiling of A. baumannii. This analysis represents a promising starting point for further investigations on the biological role of phosphorylation in A. baumannii.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Acinetobacter Baumannii 1656-2

SUBMITTER: Julie Hardouin  

LAB HEAD: Julie Hardouin

PROVIDER: PXD027208 | Pride | 2022-02-17

REPOSITORIES: Pride

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Phosphorylation of Extracellular Proteins in <i>Acinetobacter baumannii</i> in Sessile Mode of Growth.

Massier Sébastien S   Robin Brandon B   Mégroz Marianne M   Wright Amy A   Harper Marina M   Hayes Brooke B   Cosette Pascal P   Broutin Isabelle I   Boyce John D JD   Dé Emmanuelle E   Hardouin Julie J  

Frontiers in microbiology 20211001

<i>Acinetobacter baumannii</i> is a problematic nosocomial pathogen owing to its increasing resistance to antibiotics and its great ability to survive in the hospital environment, which is linked to its capacity to form biofilms. Structural and functional investigations of post-translational modifications, such as phosphorylations, may lead to identification of candidates for therapeutic targets against this pathogen. Here, we present the first S/T/Y phosphosecretome of two <i>A. baumannii</i> s  ...[more]

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