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Proteomic Analysis of Mkrn3 WT vs KO mouse brain

ABSTRACT: Mutations in the E3 ubiquitin ligase Mkrn3 are associated with precocious puberty in humans. In order to determine the targets of Mkrn3, we performed a TMT-based proteomic analysis of Mkrn3 WT vs KO mouse brains.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

DISEASE(S): Central Precocious Puberty

SUBMITTER: Brandon Gassaway

LAB HEAD: Steven Gygi

PROVIDER: PXD027220 | Pride | 2023-07-20

REPOSITORIES: Pride

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MKRN3 inhibits puberty onset via interaction with IGF2BP1 and regulation of hypothalamic plasticity.

Naulé Lydie L Mancini Alessandra A Pereira Sidney A SA Gassaway Brandon M BM Lydeard John R JR Magnotto John C JC Kim Han Kyeol HK Liang Joy J Matos Cynara C Gygi Steven P SP Merkle Florian T FT Carroll Rona S RS Abreu Ana Paula AP Kaiser Ursula B UB

JCI insight 20230424 8

Makorin ring finger protein 3 (MKRN3) was identified as an inhibitor of puberty initiation with the report of loss-of-function mutations in association with central precocious puberty. Consistent with this inhibitory role, a prepubertal decrease in Mkrn3 expression was observed in the mouse hypothalamus. Here, we investigated the mechanisms of action of MKRN3 in the central regulation of puberty onset. We showed that MKRN3 deletion in hypothalamic neurons derived from human induced pluripotent s ...[more]

PMID: 37092553

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