Proteomics

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Screening of host proteins interacting with African swine fever virus MGF360-9L

ABSTRACT: To identify the potential host protein binding to African swine fever virus MGF360-9L, the 3 × FLAG tag MGF360-9L plasmid and empty FLAG were transfected into PK-15 cells for co-immunoprecipitation and LC-MS analysis. The cell lysates were immunoprecipitated using anti-FLAG antibody. Immunoprecipitation samples of cells transfected with empty FLAG were used as negative controls to eliminate non-specific interactions. LC-MS identified the interaction between MGF360-9L and PK-15 cell proteome. The final MGF360-9L binding protein data eliminated the nonspecific interaction through empty FLAG control. The interactions that remained were analyzed by significance analysis of interactome. Finally, 268 cellular proteins interacting with MGF360-9L were identified.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Cell Culture, Kidney Cell

SUBMITTER: yanzhen Zhao  

LAB HEAD: Bo Yang

PROVIDER: PXD027260 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Construction, Identification and Analysis of the Interaction Network of African Swine Fever Virus MGF360-9L with Host Proteins.

Yang Bo B   Zhang Dajun D   Shi Xijuan X   Shen Chaochao C   Hao Yu Y   Zhang Ting T   Yang Jinke J   Yuan Xingguo X   Chen Xuehui X   Zhao Dengshuai D   Cui Huimei H   Li Dan D   Zhu Zixiang Z   Tian Hong H   Yang Fan F   Zheng Haixue H   Zhang Keshan K   Liu Xiangtao X  

Viruses 20210910 9

African swine fever virus (ASFV) is prevalent in many countries and is a contagious and lethal virus that infects pigs, posing a threat to the global pig industry and public health. The interaction between the virus and the host is key to unlocking the mystery behind viral pathogenesis. A comprehensive understanding of the viral and host protein interaction may provide clues for developing new antiviral strategies. Here, we show a network of ASFV MGF360-9L protein interactions in porcine kidney  ...[more]

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