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O-Fucosylation of CPN20 by SPINDLY Derepresses Abscisic Acid Signaling during Seed Germination and Seedling Development--Partial Submission

ABSTRACT: We utilized ETD (Electron Transfer Dissociation)-MS/MS analysis to identify O-fucosylation sites in ectopically expressed CPN20 in mammalian cells and Arabidopsis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Wen Zhou

LAB HEAD: Wen Zhou

PROVIDER: PXD027272 | Pride | 2022-02-17

REPOSITORIES: Pride

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O-fucosylation of CPN20 by SPINDLY Derepresses Abscisic Acid Signaling During Seed Germination and Seedling Development.

Liang Lin L Wang Qi Q Song Zihao Z Wu Yaxin Y Liang Qing Q Wang Qingsong Q Yang Jinli J Bi Ying Y Zhou Wen W Fan Liu-Min LM

Frontiers in plant science 20211012

SPINDLY is involved in some aspects of plant development. However, the nature of this protein as an O-fucosyltransferase was recently discovered. In this study, we show that SPINDLY (SPY) interacts with CPN20 in yeast two-hybrid and split-luc assays, and the interaction is promoted by ABA. CPN20 is a chloroplast-localized co-chaperonin that negatively regulates ABAR-mediated ABA signaling. By using Electron Transfer Dissociation-MS/MS analysis, two O-fucosylation site ...[more]

PMID: 34712252

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