Proteomic profiling of glioblastoma stem cells, human astrocyte, and T98G glioblastoma cell line

ABSTRACT: With HiRIEF LC-MS/MS shotgun proteomics, we analysed 6 patient-derived glioblastoma stem cells (BT stem cells) and compared them to an astrocyte line (CliniSciences, Guidonia Montecelio, Italy) and a more differentiated glioblastoma cell line (T98G). Each of the 8 cell line sample was run in triplicates in a total of three TMT10 sets, assigning each replicate in a separate TMT10 set, using 2 internal standards per set. The three TMT10 sets were ran in two experiments, first on immobilized pH gradient (IPG) 3-10 isoelectric focusing (IEF) strips, and then on IPG 3.7-4.9 IEF strips.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Malignant Cell, Astrocyte Cell Line, Glioblastoma Multiforme Cancer Stem Cell, Astrocyte, Glioblastoma Cell Line

DISEASE(S): Glioblastoma,Glioblastoma Classical Subtype,Brain Cancer,Idh-wildtype Glioblastoma

SUBMITTER: Haris Babačić  

LAB HEAD: Janne Lehtiö

PROVIDER: PXD027335 | Pride | 2022-12-23

REPOSITORIES: Pride