Identification of protein-protein interactions in live cells using photoactivatable myristate probe X10

ABSTRACT: 19 TST-tagged myristoylated proteins (baits) were transfected into cells and labeled with photoactivatable clickable myristate probe X10. mCitrine-TST was used as a negative control. X10-labeled proteins were photocrosslinked in live cells to their interacting partners with the aim of identifying new PTM-mediated protein-protein interactions. Proteins enriched with a certain TST-tagged bait were considered interactors when compared to the presence in all other baits, with statistical significance FDR<0.05.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Roman Fedoryshchak  

LAB HEAD: Edward W Tate

PROVIDER: PXD027394 | Pride | 2023-02-04

REPOSITORIES: Pride