Proteomics

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Evaluation of burbot Lota lota spermatozoon motility, lipid composition, protein profile, and swelling rate following change of temperature and osmolality


ABSTRACT: Despite available information on the roles of osmotic pressure, potassium (K+), and calcium (Ca2+) in activation of Eurasian burbot spermatozoon motility, the changes in sperm biochemical composition and mechanisms underlying their activation at temperatures above optimal spawning temperature is still unclear. We explored sperm swelling, lipid composition, and proteome in relation to osmolality and temperature of swimming medium. Higher temperatures increase burbot spermatozoa vulnerability to osmotic pressure, decreasing motility in extremely hypotonic media, and the presence of Ca2+ decreases swelling of spermatozoa. A role of swelling in activation of spermatozoa motility at high temperature was rejected. We observed no differences in protein profile or lipid composition with respect to activation of burbot spermatozoa at high temperature. Burbot sperm activation occurs at higher temperatures without modification of the spermatozoon membrane. Elucidation of the intrinsic signaling pathway of burbot spermatozoon spontaneous activation requires further study.

INSTRUMENT(S): autoflex

ORGANISM(S): Lota Lota

TISSUE(S): Spermatozoon

SUBMITTER: Dmitry Loginov  

LAB HEAD: Jan Sterba

PROVIDER: PXD027402 | Pride | 2022-09-20

REPOSITORIES: Pride

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