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Rat cell supernatant and the separation of supernatant LC-MSMS

ABSTRACT: We find one organ of body is special useful for skeletal muscle recovery from indury. To identify the active substance in TCM(special organ cell culturing supernatant which can activate satellite cell proliferation), we prepared serum-free TCM and also separate several fractions, subjected them to LC-MS/MS analysis. To our surprise, most proteins extracted belong to metabolite interconversion enzymes, nucleic acid binding proteins, cytoskeletal proteins, protein modifying enzymes, membrane traffic proteins and so on, but no cytokine was detected.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Cell Culture

SUBMITTER: zhang xuena

LAB HEAD: Zhang Xuena

PROVIDER: PXD027407 | Pride | 2022-02-17

REPOSITORIES: Pride

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			Action	DRS
	6.wiff	Wiff
	6.wiff.scan	Wiff
	6_PeptideSummary.txt	Txt
	6_ProteinSummary.txt	Txt
	total-1.wiff	Wiff

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Publications

The thymus regulates skeletal muscle regeneration by directly promoting satellite cell expansion.

Zheng Yan-Yan YY Wang Ye Y Chen Xin X Wei Li-Sha LS Wang Han H Tao Tao T Zhou Yu-Wei YW Jiang Zhi-Hui ZH Qiu Tian-Tian TT Sun Zhi-Yuan ZY Sun Jie J Wang Pei P Zhao Wei W Li Ye-Qiong YQ Chen Hua-Qun HQ Zhu Min-Sheng MS Zhang Xue-Na XN

The Journal of biological chemistry 20211220 1

The thymus is the central immune organ, but it is known to progressively degenerate with age. As thymus degeneration is paralleled by the wasting of aging skeletal muscle, we speculated that the thymus may play a role in muscle wasting. Here, using thymectomized mice, we show that the thymus is necessary for skeletal muscle regeneration, a process tightly associated with muscle aging. Compared to control mice, the thymectomized mice displayed comparable growth of muscle mass, but decreased muscl ...[more]

PMID: 34942145

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