Proteomics

Dataset Information

A Data-Dependent Acquisition Ladder for Ultrasensitive (Neuro)Proteomics


ABSTRACT: We report here a data acquisition strategy that expands the detectable and quantifiable proteome from trace amounts of protein digests using microanalytical capillary electrophoresis (µCE) electrospray ionization (ESI) high-resolution mass spectrometry (HRMS). Data-dependent acquisition (DDA) was programmed to progressively exclude high-intensity peptide signals from repeated analysis of the sample, essentially forming a ladder of DDA measurements.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Peter Nemes  

LAB HEAD: Peter Nemes

PROVIDER: PXD027428 | Pride | 2022-02-18

REPOSITORIES: Pride

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