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Interactome analyses reveal profound proteasome structural and functional rearrangements throughout mammalian spermatogenesis

ABSTRACT: Bottom-up, top-down proteomics and HDX-MS analysis of spermatoproteasome immunopurified from bovin testes and/or rat germ cells.

INSTRUMENT(S): Synapt MS, Orbitrap Fusion

ORGANISM(S): Rattus Norvegicus (rat) Bos Taurus (bovine)

TISSUE(S): Testis

SUBMITTER: Julien Marcoux

LAB HEAD: Julien Marcoux

PROVIDER: PXD027436 | Pride | 2022-04-07

REPOSITORIES: Pride

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Proteasome complexes experience profound structural and functional rearrangements throughout mammalian spermatogenesis.

Živković Dušan D Sanchez Dafun Angelique A Menneteau Thomas T Schahl Adrien A Lise Sandrine S Kervarrec Christine C Toste Rêgo Ana A da Fonseca Paula C A PCA Chavent Matthieu M Pineau Charles C Burlet-Schiltz Odile O Marcoux Julien J Bousquet Marie-Pierre MP

Proceedings of the National Academy of Sciences of the United States of America 20220404 15

During spermatogenesis, spermatogonia undergo a series of mitotic and meiotic divisions on their path to spermatozoa. To achieve this, a succession of processes requiring high proteolytic activity are in part orchestrated by the proteasome. The spermatoproteasome (s20S) is specific to the developing gametes, in which the gamete-specific α4s subunit replaces the α4 isoform found in the constitutive proteasome (c20S). Although the s20S is conserved across species and was shown to be crucial for ge ...[more]

PMID: 35377789

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