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Proteomic and phosphoproteomic profiling of aging human mammary myoepithelial and luminal epithelial cells

ABSTRACT: The aim of this project is to examine age-dependent changes in the proteomes and phosphoproteomes of normal human mammary luminal epithelial and myoepithelial cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Breast Epithelium, Breast

SUBMITTER: Stefan Hinz

LAB HEAD: Mark Labarge

PROVIDER: PXD027540 | Pride | 2021-11-03

REPOSITORIES: Pride

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Deep proteome profiling of human mammary epithelia at lineage and age resolution.

Hinz Stefan S Manousopoulou Antigoni A Miyano Masaru M Sayaman Rosalyn W RW Aguilera Kristina Y KY Todhunter Michael E ME Lopez Jennifer C JC Sohn Lydia L LL Wang Leo D LD LaBarge Mark A MA

iScience 20210823 9

Age is the major risk factor in most carcinomas, yet little is known about how proteomes change with age in any human epithelium. We present comprehensive proteomes comprised of >9,000 total proteins and >15,000 phosphopeptides from normal primary human mammary epithelia at lineage resolution from ten women ranging in age from 19 to 68 years. Data were quality controlled and results were biologically validated with cell-based assays. Age-dependent protein signatures were identified using differe ...[more]

PMID: 34522866

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