Dataset Information

Human monocyte-derived macrophages LC-MS/MS

ABSTRACT: We utilized proteomics analysis to ascertain the protein expression profile regulated by MALAT1 of macrophages and find out the protein network of MALAT1 regulation in human macrophages.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Macrophage

SUBMITTER: meirong wang

LAB HEAD: Wei Hou

PROVIDER: PXD027570 | Pride | 2025-07-14

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	YF_humancell_3iTRAQ_f1.wiff	Wiff
	YF_humancell_3iTRAQ_f1.wiff.scan	Wiff
	YF_humancell_3iTRAQ_f10.wiff	Wiff
	YF_humancell_3iTRAQ_f10.wiff.scan	Wiff
	YF_humancell_3iTRAQ_f11.wiff	Wiff

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Publications

LncRNA MALAT1 Facilitates HIV-1 Replication by Upregulation of CHCHD2 and Downregulation of IFN-I Expression.

Wang Mei-Rong MR Bai Cheng-Si CS Dai Jian-Wei JW Yang Lan L Quan Fang-Yi FY Ma Jian-Chun JC Chen Xing-Yuan XY Zhu Shao-Wei SW Xu Ying-Qi YQ Xiang Zhou-Fu ZF Jiang Ya-le YL Cheng Qi Q Zhang Wei-Hao WH Chen Ke-Han KH Wang Jian-Hua JH Feng Yong Y Chen Xiao-Ping XP Xiong Yong Y Chen Shu-Liang SL Hou Wei W Xiong Hai-Rong HR

Molecular & cellular proteomics : MCP 20250523 7

Long noncoding RNAs (lncRNAs) are effective regulators of both RNA and protein functions throughout cell biology, including viral replication. Emerging studies have shown that lncRNAs activate or inhibit the replication and latency of HIV-1 by regulating different cellular mechanisms. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is an oncogenic lncRNA required for paraspeckle integrity and has been proven to be linked to viral infection. However, the mechanisms by which it inf ...[more]

PMID: 40414289

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Project description:Background: Mycobacterium avium subspecies paratuberculosis (MPTb) is the causative agent of Johne’s disease, an intestinal disease of ruminants with major economic consequences. MPTb bacilli are phagocytosed by host macrophages upon exposure where they persist, resulting in lengthy subclinical phases of infection that can lead to immunopathology and disease dissemination. Consequently, analysis of the macrophage transcriptome in response to MPTb infection can provide valuable insights into the molecular mechanisms that underlie Johne’s disease. Here, we investigate pan-genomic gene expression in bovine monocyte-derived macrophages (MDM) purified from seven age-matched non-related females, in response to in vitro infection with MPTb (multiplicity of infection 2:1) at intervals of 2 hours, 6 hours and 24 hours post-infection. Results: Differentially expressed genes were identified by comparing the transcriptomes of the infected MDM to the control MDM at each time point (adjusted P-value threshold ≤ 0.10). 1,050 differentially expressed unique genes were identified 2 hours post-infection, with 974 and 84 differentially expressed unique genes detected 6 hours and 24 hours post-infection, respectively. Furthermore, in the infected MDM the number of upregulated genes exceeded the number of downregulated genes at each time point, with the fold-change in expression for the upregulated genes markedly higher than that for the downregulated genes. Conclusions: Inspection and systems analysis of the differentially expressed genes revealed changes in the expression profiles of genes involved in the inflammatory response, cell signalling pathways and apoptosis. The transcriptional changes associated with cellular signalling and the inflammatory response may reflect different immuno-modulatory mechanisms that underlie host-pathogen interactions during infection. Affymetrix GeneChip® Bovine Genome Arrays were used to examine gene expression of bovine monocyte-derived macrophages (MDM) after in vitro challenge with Mycobacterium avium subspecies paratuberculosis across a time series of 2 hr, 6 hr and 24 hr post-challenge. A 0 hr control treatment was also generated and seven different age-matched female Holstein-Friesian cattle were used for each time-point/treatment combination for a total of 49 microarrays, one of which was excluded from analysis since it did not pass quality control.