Proteomics

Dataset Information

0

LC-MS/MS data of Extracellular Vimentin

ABSTRACT: Angiotensin-converting enzyme 2 (ACE2) has been identified as a key entry receptor for SARS-CoV-2. The involvement of other cellular components that partake in viral entry appears to be likely but is not yet fully understood. Here we describe the identification of vimentin (VIM), an intermediate filament protein widely expressed in cells of mesenchymal origin, as an important attachment factor for SARS-CoV-2 on human endothelial cells. Using liquid chromatography–tandem mass spectrometry (LC-MS/MS), we identified VIM as a protein that binds to the SARS-CoV-2 spike (S) protein.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endothelial Cell

SUBMITTER: Chaoshuang Xia  

LAB HEAD: Catherine E. Costello

PROVIDER: PXD027595 | Pride | 2022-02-22

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
103020_Control_Binding_fraction_90min_method.raw Raw
103020_Control_washing_fraction_90min_method.raw Raw
103020_IP_Binding_fraction_90min_method_20201030174033.raw Raw
103120_IP_washing_fraction_90min_method.raw Raw
20201215_ControlSample_B1.raw Raw
Items per page:
1 - 5 of 14
altmetric image

Publications

Extracellular vimentin is an attachment factor that facilitates SARS-CoV-2 entry into human endothelial cells.

Amraei Razie R   Xia Chaoshuang C   Olejnik Judith J   White Mitchell R MR   Napoleon Marc A MA   Lotfollahzadeh Saran S   Hauser Blake M BM   Schmidt Aaron G AG   Chitalia Vipul V   Mühlberger Elke E   Costello Catherine E CE   Rahimi Nader N  

Proceedings of the National Academy of Sciences of the United States of America 20220201 6

SARS-CoV-2 entry into host cells is a crucial step for virus tropism, transmission, and pathogenesis. Angiotensin-converting enzyme 2 (ACE2) has been identified as the primary entry receptor for SARS-CoV-2; however, the possible involvement of other cellular components in the viral entry has not yet been fully elucidated. Here we describe the identification of vimentin (VIM), an intermediate filament protein widely expressed in cells of mesenchymal origin, as an important attachment factor for S  ...[more]

Similar Datasets

Transcriptomics SUMOylation restrains the degradation of ACE2 through TOLLIP-mediated selective autophagy to facilitate the host susceptibility to SARS-CoV-2 infection
2022-07-18 | GSE171130 | GEO
Transcriptomics ACE2-independent SARS-CoV-2 virus entry through cell surface GRP78 on monocytes - evidence from a translational clinical and experimental approach
2023-12-13 | GSE245922 | GEO
Transcriptomics Kinase-independent activity of DYRK1A promotes viral entry of highly pathogenic human coronaviruses [RNAseq]
2023-04-02 | GSE213998 | GEO
Genomics Kinase-independent activity of DYRK1A promotes viral entry of highly pathogenic human coronaviruses [ATACseq]
2023-04-02 | GSE213997 | GEO
Transcriptomics Measuring the mutation rate of the developed trVLP-ASV system
2025-08-06 | GSE302091 | GEO
Transcriptomics Screening for SA55 escape mutations using a DMS library based on trVLP-ASV
2025-08-06 | GSE302090 | GEO
Transcriptomics Identifying antibody escape mutations using the evolutionary capacity of trVLP-ASV
2025-08-06 | GSE302087 | GEO
Transcriptomics Single-cell sequencing of glioblastoma reveals central nervous system susceptibility to SARS-CoV-2
2020-11-12 | GSE157424 | GEO
Transcriptomics SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues
2020-04-23 | GSE148829 | GEO
Transcriptomics ACE2 knockout hinders SARS-CoV-2 propagation in iPS cell-derived airway and alveolar epithelial cells
2024-08-21 | GSE275240 | GEO