Proteomics

Dataset Information

0

Global and mitochondrial proteomics under GSH depletion


ABSTRACT: Glutathione (GSH) is a tripeptide thiol abundantly present in all eukaryotes1. As the major site of oxidative metabolism, mitochondria must maintain sufficient levels of GSH in order to perform key protective and biosynthetic functions. GSH is exclusively synthesized in the cytosol, yet how it enters mitochondria and the molecular machinery involved in mitochondrial GSH import remain elusive. Here, using mass-spectrometry approaches, we identify SLC25A39, a mitochondrial membrane carrier of unknown function, to mediate GSH transport into mitochondria. SLC25A39 loss reduces mitochondrial GSH import and abundance, without impacting whole cell GSH levels.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Soren Heissel  

LAB HEAD: Kivanc Birsoy

PROVIDER: PXD027673 | Pride | 2021-11-15

REPOSITORIES: Pride

Similar Datasets

2019-03-16 | GSE96727 | GEO
2019-03-16 | GSE96726 | GEO
2017-10-08 | GSE62170 | GEO
2023-10-28 | GSE160355 | GEO
2018-04-20 | GSE107784 | GEO
2022-08-04 | GSE207212 | GEO
2014-08-01 | E-GEOD-57596 | biostudies-arrayexpress
2023-03-17 | GSE227295 | GEO
2023-10-08 | GSE236975 | GEO
2022-10-04 | GSE198540 | GEO