Proteomics

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A deeper look at carrier proteome effects for single-cell proteomics


ABSTRACT: We probe the carrier proteome effects in single cell proteomics with mixed species TMTpro-labeled samples. We demonstrate that carrier proteomes, while increasing overall identifications, dictate which proteins are identified. We show that quantitative precision and signal intensity are limited at high carrier levels, hindering the recognition of regulated proteins. Guidelines for optimized mass spectrometry acquisition parameters and best practices for fold-change or protein copy number-based comparisons are provided.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human) Escherichia Coli Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Zilu Ye  

LAB HEAD: Jesper V. Olsen

PROVIDER: PXD027742 | Pride | 2022-02-24

REPOSITORIES: Pride

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