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PChem_benchmarking_dataset_redoxome

ABSTRACT: Data sets are used for benchmarking, pChem, a modification-centric, blind-search tool to provide a streamlined pipeline for unbiased assessing of the performance of chemoproteomic probes. Data were produced from the redoxome profiling experiments using oxoform-specific probes as indicated, including SOH probes (DYn-2, BTD, WYneC/O/N) and SO2H probe (DiaAlk). All these probes contain a clickable alkyne handle.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Jing Yang

LAB HEAD: Jing Yang

PROVIDER: PXD027764 | Pride | 2023-02-27

REPOSITORIES: Pride

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A modification-centric assessment tool for the performance of chemoproteomic probes.

He Ji-Xiang JX Fei Zheng-Cong ZC Fu Ling L Tian Cai-Ping CP He Fu-Chu FC Chi Hao H Yang Jing J

Nature chemical biology 20220721 8

Chemoproteomics has emerged as a key technology to expand the functional space in complex proteomes for probing fundamental biology and for discovering new small-molecule-based therapies. Here we report a modification-centric computational tool termed pChem to provide a streamlined pipeline for unbiased performance assessment of chemoproteomic probes. The pipeline starts with an experimental setting for isotopically coding probe-derived modifications that can be automatically recognized by pChem ...[more]

PMID: 35864333

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