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A proteomic approach for unravelling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica survival and virulence

ABSTRACT: Please provide an overall description of your study, think something similar in scope to the manuscript abstractCFIm25 is essential for recruitment of other polyadenylation factors, poly(A) site selection, pre-mRNA cleavage and poly(A) tail synthesis. We recently reported that EhCFIm25 silencing in Entamoeba histolytica, the protozoan responsible for amebic dysentery, affects trophozoite proliferation and death, and reduces parasite virulence, indicating its relevance as a new biochemical target in this human pathogen. Here, we performed a shotgun proteomic analysis to evidence the impact of EhCFIm25 silencing on protein expression that can explain the observed phenotype

INSTRUMENT(S): Synapt G2 MS

ORGANISM(S): Entamoeba Histolytica Hm-1:imss-a

SUBMITTER: Rodolfo G Avila-Bonilla

LAB HEAD: Laurence A. Marchat

PROVIDER: PXD027784 | Pride | 2021-08-26

REPOSITORIES: Pride

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A folded conformation of MukBEF and Cohesin

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Transcription profiling of primary human astrocytes from brain treated by stathmin, poly IC and ultrapure LPS to examine the effects of different Toll-like receptor agonists

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Unbiased screen of RNA tailing enzymes reveals a poly(UG) polymerase required for genome integrity and RNA silencing

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2018-12-08 | GSE123478 | GEO

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