Proteomics

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The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-male isotopic labeling to identify female-derived sperm proteins

ABSTRACT: Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to identify female-derived proteins that contribute to the sperm proteome after long-term storage in the female’s sperm storage organs. In combination with whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days), as well as an expansion of the seminal vesicle sperm proteome, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Spermatozoon

SUBMITTER: Erin McCullough  

LAB HEAD: Steve Dorus

PROVIDER: PXD027802 | Pride | 2022-03-11

REPOSITORIES: Pride

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The life history of <i>Drosophila</i> sperm involves molecular continuity between male and female reproductive tracts.

McCullough Erin L EL   Whittington Emma E   Singh Akanksha A   Pitnick Scott S   Wolfner Mariana F MF   Dorus Steve S  

Proceedings of the National Academy of Sciences of the United States of America 20220307 11

SignificanceIn species with internal fertilization, sperm spend an important part of their lives within the female. To examine the life history of the sperm during this time, we used semiquantitative proteomics and sex-specific isotopic labeling in fruit flies to determine the extent of molecular continuity between male and female reproductive tracts and provide a global catalog of sperm-associated proteins. Multiple seminal fluid proteins and female proteins associate with sperm immediately aft  ...[more]

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