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The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts

ABSTRACT: Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days). In combination with an update and expansion of the seminal vesicle sperm proteome and sex-specific isotopic labeling to identify female-contributed sperm proteins, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Spermatozoon

SUBMITTER: Erin McCullough

LAB HEAD: Steve Dorus

PROVIDER: PXD027807 | Pride | 2022-03-14

REPOSITORIES: Pride

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			Action	DRS
	AkankshaSingh10356322_HighMW_N1.mgf	Mgf
	AkankshaSingh10356322_HighMW_N1.raw	Raw
	AkankshaSingh10356322_LowMW_N4.mgf	Mgf
	AkankshaSingh10356322_LowMW_N4.raw	Raw
	AkankshaSingh10356322_MidMW_N3.mgf	Mgf

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Publications

Bone marrow transplantation.

Kay H E HE

British journal of haematology 19761201 4

PMID: 10959

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The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-male isotopic labeling to identify female-derived sperm proteins

Project description:Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to identify female-derived proteins that contribute to the sperm proteome after long-term storage in the female’s sperm storage organs. In combination with whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days), as well as an expansion of the seminal vesicle sperm proteome, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

2022-03-11 | PXD027802 | Pride

The life history of Drosophila sperm depends on molecular continuity between male and female reproductive tracts: heavy-female isotopic labeling to estimate abundances of female-derived sperm protein

Project description:Interactions between sperm and the female reproductive tract (FRT) are critical to fertility, but knowledge of the molecular mechanisms by which the FRT interacts with sperm and influences sperm fate remains limited. Here, we used sex-specific isotopic labeling to estimate the relative abundance of female-derived sperm proteins after long-term storage in the female’s sperm storage organs. In combination with whole-cell quantitative proteomics to track the protein composition of sperm across three female reproductive tissues (bursa, seminal receptacle, and spermatheca) and three post-mating timepoints (30 minutes, 2 hours, and 4 days), as well as an expansion of the seminal vesicle sperm proteome, our data provide a comprehensive, quantitative analysis of the molecular life history of Drosophila sperm.

2022-03-14 | PXD027806 | Pride

Seminal fluid and sperm proteomics of Aedes aegypti

Project description:Sperm samples were extracted from adult A. aegypti male seminal vesicles. Semen samples were extracted from bursa of recently mated bursa of N15 labeled females. The goal was to differentiate between the contribution of seminal fluid proteins and sperm proteins in the semen transfered to the female. Our results yield insights into the molecular function, genome organization, regulation, and evolution of sperm proteins and SFPs in this important disease vector.

2018-12-17 | PXD010293 | Pride

Proteomic differences between Drosophila melanogaster female reproductive tract tissue and fluid and their postmating responses

Project description:Fertility depends on the progression of complex and coordinated postmating processes within the extracellular luminal environment of the female reproductive tract (FRT). To achieve a more comprehensive level of knowledge regarding female-derived proteins available to interact with the ejaculate, we utilized semiquantitative mass spectrometry-based proteomics to study the composition of the FRT tissue and, separately, the luminal fluid, before and after mating in Drosophila melanogaster. Our approach leveraged whole-fly isotopic labelling to delineate between female proteins and those transferred from males in the ejaculate. The dynamic mating-induced proteomic changes in the extracellular FRT luminal fluid further informs our understanding of secretory mechanisms of the FRT and serves as a foundation for establishing the roles of ejaculate-female interactions in fertility.

2021-09-22 | PXD025085 | Pride

Expansion of the Drosophila melanogaster sperm proteome

Project description:Given the continued advances in mass spectrometry technology and methods for database searching since the previous characterization of the Drosophila melanogaster sperm proteome, a new proteomic analysis of sperm samples was conducted to expand the size and coverage of the sperm proteome. This dataset is part of a larger project examining the molecular life history of Drosophila sperm.

2022-03-14 | PXD027808 | Pride

Comparison of post-mating responses in the female reproductive tract among Drosophila species

Project description:Fertility depends on the coordination of complex interactions between male and female reproductive proteins inside the female reproductive tract (FRT). These interactions mediate a suite of changes in female behavior, morphology, and physiology after mating, yet little is known about how the molecular environment of the FRT may differ among species and coordinate species-specific female post-mating responses. We used semi-quantitative proteomics to compare the FRT protein composition between virgin and mated females in Drosophila melanogaster. These results are compared to those from quantitative TMT proteomic analyses of the mating-induced changes in D. simulans and D. mauritiana, and after conspecific and heterospecific inseminations. Our study highlights the value of using quantitative proteomics approaches to study the molecular composition of the FRT environment, and how its divergence may inform mechanistic studies of post-mating pre-zygotic reproductive isolation between species.

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Mating and sperm free seminal plasma changes gene expression in the oviductal sperm reservoirs of sows

Project description:Sperms being foreign to the female are to be promptly eliminated by the female local immune defense. However, avoiding the local immune defense sperm can be stored for lenthy period in the oviductal sperm reservoir. It is currently unknown whether oviductal sperm reservoirs changes their gene expression to tolerate the spermatozoa after mating or sperm free seminal plasma infusion. Therefore this was tested using Swedish Landrace pigs in this study using cDNA microarray. We used 12 sows seperated into three groups- either oestrus sows were inseminated with 50 ml BTS (control, n=4) or mated with boars (treatment 1, n=4) or inseminated with sperm-free seminal plasma (treatment 2, n=4). The utero-tubal junction was retrieved within 24 h of treatment by operation.

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Project description:Comparison of females mated to males null for Sex-Peptide (SP0, Liu, H. and E. Kubli, Sex-peptide is the molecular basis of the sperm effect in Drosophila melanogaster. Proc Natl Acad Sci U S A, 2003. 100(17): p. 9929-33) or to control, Sex-Peptide producing, males. Comparisons were made at 3 and 6 hours after mating, in dissected Head-Thorax body parts.

2009-08-01 | E-MEXP-2070 | biostudies-arrayexpress

Transcription profiling of Drosophila abdomen from females mated with sex peptide null or sex peptide producing males

2009-08-01 | E-MEXP-2074 | biostudies-arrayexpress

Transcription profiling of sperm storage organs (spermathecae) from female Anopheles gambiae mosquitoes as virgin and 24 hours after mating

Project description:Since Anopheles gambiae female mosquitoes mate only once in their lifetime, and store sperm received from males potentially for weeks, genes and pathways regulated by mating in the sperm storage organ (spermatheca) to preserve sperm function may represent new targets for vector control. The transcriptional response to mating in the spermatheca was investigated using Agilent two-color microarrays. Spermathecae from mated females were collected 24 h after mating and compared to age-matched virgins. 4 biological replicates were performed.

2014-09-18 | E-MTAB-2429 | biostudies-arrayexpress

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