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Label-free LC-MS/MS of Ubiquitinated proteins derived from UPs-4T1/WT and UPs-4T1/EPB nanovaccine

ABSTRACT: In this project, 4T1 parental cells (4T1/WT) were exposed to increasing concentrations of epirubicin (EPB) to establish a novel multi-drug resistant CSC-like breast cancer cell line (4T1/EPB). The ubiquitinated proteins were enriched from 4T1/WT or 4T1/EPB derived cell lysate using a-Al2O3-Vx3 nanoparticles to produce the covalently linked product UPs nanovaccine. Label-free LC-MS/MS mass spectrometry was used to detect the type and amount of enriched proteins of UPs from the 4T1/WT cells and the 4T1/EPB cells.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stem Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Fang Huang

LAB HEAD: Fang Huang

PROVIDER: PXD027822 | Pride | 2021-08-20

REPOSITORIES: Pride

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