Dataset Information

Differential bottom-up proteomics of truffles

ABSTRACT: To compare truffles on protein level, samples of different truffle species (T. magnatum, T. indicum, T. melanosporum, T. aestivum and T. albidum Pico) were analysed by a LC-MS/MS based shotgun proteomics approach. First, different established bottom-up proteomics sample preparation approaches for in solution tryptic proteolysis were tested for a truffle sample. Then, a spectral library was generated by 2D LC-MS/MS and truffle samples were measured in data-independent acquisition (DIA) mode. We provide a comprehensive study on the proteomic differences between truffle species.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Tuber Albidum Tuber Magnatum Tuber Aestivum Tuber Melanosporum Mel28 Tuber Indicum

TISSUE(S): Fruit Body

SUBMITTER: Dennis Krösser

LAB HEAD: Hartmut Schlüter

PROVIDER: PXD027871 | Pride | 2021-12-19

REPOSITORIES: Pride

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Publications

Investigation of the Proteomes of the Truffles Tuber albidum pico, T. aestivum, T. indicum, T. magnatum, and T. melanosporum.

Krösser Dennis D Dreyer Benjamin B Siebels Bente B Voß Hannah H Krisp Christoph C Schlüter Hartmut H

International journal of molecular sciences 20211130 23

Truffles of the Tuber species are known as expensive foods, mainly for their distinct aroma and taste. This high price makes them a profitable target of food fraud, e.g., the misdeclaration of cheaper truffle species as expensive ones. While many studies investigated truffles on the metabolomic level or the volatile organic compounds extruded by them, research at the proteome level as a phenotype determining basis is limited. In this study, a bottom-up proteomic approach based on LC-MS/MS ...[more]

PMID: 34884803

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Dataset Information

Differential bottom-up proteomics of truffles

Publications

Investigation of the Proteomes of the Truffles <i>Tuber albidum pico</i>, <i>T. aestivum</i>, <i>T. indicum</i>, <i>T. magnatum</i>, and <i>T. melanosporum</i>.

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