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Aspirin Acetylates Thioredoxin Reductase 1 (isoform 4)

ABSTRACT: Thioredoxin Reductase 1 (isoform 4) was incubated with aspirin for 1 hour at 37 C followed by SDS PAGE. Samples were picked from SDS PAGE gel, digested with trypsin, and sent for LC-MS/MS.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: David Wright

LAB HEAD: Patrick O'Donoghue

PROVIDER: PXD027882 | Pride | 2022-02-17

REPOSITORIES: Pride

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Acetylated Thioredoxin Reductase 1 Resists Oxidative Inactivation.

Wright David E DE Panaseiko Nikolaus N O'Donoghue Patrick P

Frontiers in chemistry 20210915

Thioredoxin Reductase 1 (TrxR1) is an enzyme that protects human cells against reactive oxygen species generated during oxidative stress or in response to chemotherapies. Acetylation of TrxR1 is associated with oxidative stress, but the function of TrxR1 acetylation in oxidizing conditions is unknown. Using genetic code expansion, we produced recombinant and site-specifically acetylated variants of TrxR1 that also contain the non-canonical amino acid, selenocysteine, which is essential for TrxR1 ...[more]

PMID: 34604175

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