Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PH dependence of succinimide ester-based protein cross-linking for structural mass spectrometry applications

ABSTRACT: The pH dependency of the reaction of the cross-linking reagent, disuccinimidyl suberate (DSS), was studied on a set of eight model proteins.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine) Gallus Gallus (chicken) Oryctolagus Cuniculus (rabbit)

SUBMITTER: Alexander Leitner

LAB HEAD: Alexander Leitner

PROVIDER: PXD027891 | Pride | 2021-11-15

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	File_list.xlsx	Xlsx
	Results_lowpH.xlsx	Xlsx
	arekelle_C1910_028.raw	Raw
	arekelle_C1910_029.raw	Raw
	arekelle_C1910_032.raw	Raw

Items per page:

1 - 5 of 35

Similar Datasets

Characterization of Integrator module INTS10-INTS13-INTS14 and its connection to the cleavage module Part I

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the complex between the Integrator complex subunits INTS13 (Asunder) and INTS14. Cross-linking was performed using disuccinimidyl suberate (DSS).

2020-06-18 | PXD015682 | Pride

The TRiC Chaperonin and Prefoldin Cooperate to Fold and Assemble Viral Capsid Oligomers

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the folding of the reovirus sigma3 protein by the chaperonin, TRiC/CCT. Cross-linking was performed using the homobifunctional, noncleavable reagent, disuccinimidyl suberate (DSS).

2021-02-04 | PXD019751 | Pride

Molecular architecture of the human tRNA ligase complex

Project description:Chemical cross-linking coupled to mass spectrometry was used to study two tRNA ligase complexes, one consisting of subunits RTCB, DDX1, CGI-99, FAM98B and Ashwin (“full tRLC”) and one lacking Ashwin (“core tRLC”). Cross-linking was performed using the homobifunctional, noncleavable reagent, disuccinimidyl suberate (DSS).

2021-12-15 | PXD025662 | Pride

Characterization of Integrator module INTS10-INTS13-INTS14 and its connection to the cleavage module Part II

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the complex between the Integrator complex subunits INTS4, 9, 11, and a partial segment of INTS13 containing the C-terminal region (termed CMBM). Cross-linking was performed using disuccinimidyl suberate (DSS).

2020-06-18 | PXD017996 | Pride

A structural mechanism for PP2A-B56alpha inhibition by CIP2A

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the structure of the oncoprotein CIP2A and its interaction with the B56alpha regulatory subunit of protein phosphatase 2A. Cross-linking was performed using disuccinimidyl suberate (DSS) or a combination of pimelic dihydrazide (PDH) and the coupling reagent DMTMM.

2022-10-21 | PXD020636 | Pride

The human GID complex engages two independent modules for substrate recruitment

Project description:Chemical cross-linking coupled to mass spectrometry was used to study two different assemblies of the human E3 ubiquitin ligase complex, GID. The 5mer complex consisted of subunits MAEA, RMND5a, TWA1, WDR26, and RanBP9; the 6mer complex consisted of the subunits of the 5mer complex and the additional subunit ARMC8β. Cross-linking experiments were performed with disuccinimidyl suberate (DSS) or pimelic dihydrazide (PDH) in combination with the coupling reagent DMTMM.

2021-08-25 | PXD024822 | Pride

A precisely positioned MED12 activation helix stimulates CDK8 kinase activity

Project description:Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 8 (CDK8), cyclin-C, and subunit 12 of the Mediator complex (MED12). Cross-linking was performed using different cross-linking chemistries: (1) disuccinimidyl suberate (DSS); (2) a combination of pimelic acid dihydrazide (PDH) and the coupling reagent, DMTMM.

2020-01-16 | PXD015394 | Pride

Interactions of CDK19 with MED12 and cyclin-C assessed by chemical cross-linking mass spectrometry

Project description:Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 19 (CDK19), cyclin-C, and an N-terminal fragment of subunit 12 of the Mediator complex (MED12 1-100). Cross-linking was performed using disuccinimidyl suberate (DSS). These results were generated in the context of the study published as Klatt et al., A precisely positioned MED12 activation helix stimulates CDK8 kinase activity, Proc. Natl. Acad. Sci. USA 2020 (DOI: 10.1073/pnas.1917635117) with the associated data set PXD015394, but were not included in the article.

2020-05-18 | PXD019251 | Pride

Phosphorylation of TRAIP is essential for mitotic replisome disassembly and MiDAS

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the structure and homodimerization of the E3 ubiquitin ligase TRAIP from Xenopus laevis. The protein was cross-linked with two different concentrations of disuccinimidyl suberate (DSS).

2025-06-27 | PXD058941 | Pride

Cross-linking mass spectrometry analysis of human BCL7A and BAF47 in presence and absence of nucleosome

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the interaction between BCL7A and BAF47 and their respective interactions with nucleosomes. The two proteins were cross-linked with two different concentrations of disuccinimidyl suberate (DSS) in presence and absence of nucleosomes.

2025-04-14 | PXD054757 | Pride