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Proteomics of HS-5 and NKTert stroma cells

ABSTRACT: Comparison of the proteome of the human bone marrow stroma cell lines NKTert and HS-5.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

SUBMITTER: Frank Stein

LAB HEAD: Sascha Dietrich

PROVIDER: PXD027945 | Pride | 2022-02-17

REPOSITORIES: Pride

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Phagocytosis by stroma confounds coculture studies.

Herbst Sophie A SA Stolarczyk Marta M Becirovic Tina T Czernilofsky Felix F Liu Yi Y Kolb Carolin C Knoll Mareike M Herling Marco M Müller-Tidow Carsten C Dietrich Sascha S

iScience 20210830 9

Signals provided by the microenvironment can modify and circumvent pathway activities that are therapeutically targeted by drugs. Bone marrow stromal cell coculture models are frequently used to study the influence of the bone marrow niche on <i>ex vivo</i> drug response. Here, we show that mesenchymal stromal cells from selected donors and NKTert, a stromal cell line, which is commonly used for coculture studies with primary leukemia cells, extensively phagocytose apoptotic cells. This could le ...[more]

PMID: 34585113

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