Conformation of the AP-3 complex explains its role in cargo recruitment at the Golgi

ABSTRACT: Crosslinking mass spectrometry study of an important adapter protein complex at the Golgi AP-3. We performed crosslinking mass spectrometry study using crosslinker DSSO and analyzed the digested peptides by LC-MS. The crosslinking study provided important information the subunit connectivity of AP-3, which facilitated the structural analysis of AP-3 by cyro-EM.

INSTRUMENT(S):

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Heike Stephanowitz  

LAB HEAD: Christian Ungermann

PROVIDER: PXD028005 | Pride | 2022-02-17

REPOSITORIES: Pride