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Phosphoproteomic Responses of Distal TORC1 Target Kinases Reveal Discrete and Convergent Mechanisms that Orchestrate the Quiescence Program in Yeast

ABSTRACT: TORC1 inhibition following nutrient limitation unlocks various distally controlled kinases (e.g., Atg1, Gcn2, Slt2/Mpk1, Npr1, Rim15, and Yak1), which cooperate through poorly defined circuits to orchestrate the quiescence program. To better define the signaling landscape of the latter kinases, we used in vivo quantitative phosphoproteomics. While pinpointing known and numerous hitherto uncharted Slt2/Mpk1, Npr1, Rim15, and Yak1 effectors, our study reveals insight into the architecture of the distally controlled TORC1 kinase network.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Joern Dengjel

LAB HEAD: Joern Dengjel

PROVIDER: PXD028028 | Pride | 2022-02-08

REPOSITORIES: Pride

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Phosphoproteomic responses of TORC1 target kinases reveal discrete and convergent mechanisms that orchestrate the quiescence program in yeast.

Dokládal Ladislav L Stumpe Michael M Hu Zehan Z Jaquenoud Malika M Dengjel Jörn J De Virgilio Claudio C

Cell reports 20211201 13

The eukaryotic TORC1 kinase assimilates diverse environmental cues, including growth factors and nutrients, to control growth by tuning anabolic and catabolic processes. In yeast, TORC1 stimulates protein synthesis in response to abundant nutrients primarily through its proximal effector kinase Sch9. Conversely, TORC1 inhibition following nutrient limitation unlocks various distally controlled kinases (e.g., Atg1, Gcn2, Npr1, Rim15, Slt2/Mpk1, and Yak1), which cooperate through poorly defined ci ...[more]

PMID: 34965436

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