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Unravelling the layering of microbial community, proteins and chemical structure in aerobic granules

ABSTRACT: Nearly all bacteria live in microbial assemblages such as biofilms and granules with a continuum of chemical gradients. Each of these layers provide a niche selective for specific bacteria and functions. A gentle extraction approach based on a cation exchange resin and defined shear was employed to collect single layers in microbial granules for microbial and chemical analysis. The use of an existing cation-exchange resin for gradual biomass disintegration, combined with NGS and proteome analysis have revealed a promising approach for investigating the identity and functions in multilayered biofilm structures.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sludge Granule Bacterium 1

SUBMITTER: Jan Struckmann Poulsen

LAB HEAD: Jeppe Lund Nielsen

PROVIDER: PXD028049 | Pride | 2022-03-23

REPOSITORIES: Pride

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