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Proteomic analysis of bovine milk-derived extracellular vesicles isolated via salting-out

ABSTRACT: We developed a novel and cost-effective procedure to separate bovine milk-derived extracellular vesicles via salting-out and aimed to profile their proteomics as one important characterization in EV research.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Milk

SUBMITTER: Xiaohui Tan

LAB HEAD: Rui-Li Guan

PROVIDER: PXD028095 | Pride | 2021-11-02

REPOSITORIES: Pride

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Skimmed Bovine Milk-Derived Extracellular Vesicles Isolated <i>via</i> "Salting-Out": Characterizations and Potential Functions as Nanocarriers.

Tan Xiao-Hui XH Fang Dong D Xu Yong-De YD Nan Tie-Gui TG Song Wen-Peng WP Gu Yang-Yang YY Gu Sheng-Ji SJ Yuan Yi-Ming YM Xin Zhong-Cheng ZC Zhou Li-Qun LQ Guan Rui-Li RL Li Xue-Song XS

Frontiers in nutrition 20211028

Bovine milk-derived extracellular vesicles (BM-EVs) are recognized as promising nanoscale delivery vectors owing to their large availability. However, few isolation methods can achieve high purity and yield simultaneously. Therefore, we developed a novel and cost-effective procedure to separate BM-EVs <i>via</i> "salting-out." First, BM-EVs were isolated from skimmed milk using ammonium sulfate. The majority of BM-EVs were precipitated between 30 and 40% saturation and 34% had a relatively augme ...[more]

PMID: 34778348

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