Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Epithelial Cell, Cell Culture
DISEASE(S): Pancreatic Ductal Adenocarcinoma
SUBMITTER: Michel Nofal
LAB HEAD: Josh Rabinowitz
PROVIDER: PXD028143 | Pride | 2022-10-13
REPOSITORIES: Pride
Nofal Michel M Wang Tim T Yang Lifeng L Jankowski Connor S R CSR Hsin-Jung Li Sophia S Han Seunghun S Parsons Lance L Frese Alexander N AN Gitai Zemer Z Anthony Tracy G TG Wühr Martin M Sabatini David M DM Rabinowitz Joshua D JD
Cell systems 20211026 2
Pancreatic cancer cells with limited access to free amino acids can grow by scavenging extracellular protein. In a murine model of pancreatic cancer, we performed a genome-wide CRISPR screen for genes required for scavenging-dependent growth. The screen identified key mediators of macropinocytosis, peripheral lysosome positioning, endosome-lysosome fusion, lysosomal protein catabolism, and translational control. The top hit was GCN2, a kinase that suppresses translation initiation upon amino aci ...[more]