Dataset Information

The Parallel Reaction Monitoring-Parallel Accumulation Serial Fragmentation (prm-PASEF) Approach for Multiplexed Absolute Quantitation of Proteins in Human Plasma

ABSTRACT: In the study reported here, a prm-PASEF approach was used for multiplexed absolute quantitation of proteins in human plasma using isotope-labeled peptide standards for 125 plasma proteins, over a broad (104–106) dynamic range. Optimization of LC and MS parameters, such as accumulation time and collision energy, resulted in improved sensitivity for more than half of the targets (73 out of 125 peptides) by increasing the signal-to-noise ratio by a factor of up to 10. Forty-one peptides showed up to a 2-fold increase in sensitivity, 25 peptides showed up to a 5-fold increase in sensitivity, and 7 peptides showed up to a 10-fold increase in sensitivity).

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Alexander Brzhozovskiy

LAB HEAD: Christoph Hermann Borchers

PROVIDER: PXD028279 | Pride | 2025-04-30

REPOSITORIES: Pride

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The Parallel Reaction Monitoring-Parallel Accumulation-Serial Fragmentation (prm-PASEF) Approach for Multiplexed Absolute Quantitation of Proteins in Human Plasma.

Brzhozovskiy Alexander A Kononikhin Alexey A Bugrova Anna E AE Kovalev Grigoriy I GI Schmit Pierre-Olivier PO Kruppa Gary G Nikolaev Evgeny N EN Borchers Christoph H CH

Analytical chemistry 20220118 4

Mass spectrometry (MS)-based quantitative proteomic methods have become some of the major tools for protein biomarker discovery and validation. The recently developed parallel reaction monitoring-parallel accumulation-serial fragmentation (prm-PASEF) approach on a Bruker timsTOF Pro mass spectrometer allows the addition of ion mobility as a new dimension to LC-MS-based proteomics and increases proteome coverage at a reduced analysis time. In this study, a prm-PASEF approach was used for the mult ...[more]

PMID: 35040635

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