Proteomics

Dataset Information

0

A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples.

ABSTRACT: SARS-CoV-2 has created a global disease burden infecting >100 million humans in just over a year. In this study, we adopted a SISCAPA-based enrichment approach using anti-peptide antibodies generated against peptides from the nucleocapsid protein of SARS-CoV-2. We developed a targeted workflow in which nasopharyngeal samples were digested followed by enrichment of viral peptides using the anti-peptide antibodies and targeted parallel reaction monitoring (PRM) analysis using a high-resolution mass spectrometer. This workflow was applied to 41 RT-PCR-confirmed clinical nasopharyngeal swab samples and 30 negative samples. The SISCAPA-based platform described in the current study can serve as one of the alternative methods for SARS-CoV-2 viral detection.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

DISEASE(S): Covid-19

SUBMITTER: Akhilesh Pandey  

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD028505 | Pride | 2022-02-16

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

SARS-CoV-2, a novel human coronavirus, has created a global disease burden infecting > 100 million humans in just over a year. RT-PCR is currently the predominant method of diagnosing this viral infection although a variety of tests to detect viral antigens have also been developed. In this study, we adopted a SISCAPA-based enrichment approach using anti-peptide antibodies generated against peptides from the nucleocapsid protein of SARS-CoV-2. We developed a targeted workflow in which nasopharyn  ...[more]

Similar Datasets

Proteomics A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples.
2022-06-03 | PXD026273 | Pride
Transcriptomics Trancriptome analysis of peripheral leukocytes from SARS-CoV-2 infected patients and healthy uninfected individuals during the first wave of COVID-19 pandemic in Brazil
2022-04-29 | E-MTAB-11240 | biostudies-arrayexpress
Transcriptomics Host nasopharyngeal transcriptome analysis in a SARS-CoV-2 positive Italian cohort
2023-05-25 | E-MTAB-13028 | biostudies-arrayexpress
Proteomics Detection of SARS-CoV-2 in nasal swabs using MALDI-MS
2020-09-11 | PXD021388 | Pride
Proteomics A mass spectrometry-based targeted assay for detection of SARS-CoV-2 antigen from clinical specimens
2020-08-13 | PXD020644 | Pride
Proteomics Targeted Detection of SARS-CoV-2 Protein Variants by Mass Spectrometry
2022-02-17 | PXD026795 | Pride
Proteomics Proteomic Investigation Reveals Dominant Alterations of Neutrophil Degranulation and mRNA Translation Pathways in COVID-19 Patients
2021-02-09 | PXD020580 | Pride
Transcriptomics Diagnostic Microbiology & Infectious Disease Rapid and sensitive detection of Sars-CoV-2 variants in nasopharyngeal swabs and wastewaters
2022-06-03 | E-MTAB-11261 | biostudies-arrayexpress
Transcriptomics RNA-seq of longitudinal human blood and nose swab samples from a controlled human infection experiment with SARS-CoV-2 virus
2024-04-08 | E-MTAB-12993 | biostudies-arrayexpress
Genomics Capturing SARS-cov in low abundant patient samples: a comparative analysis (metagenomics NS).
2022-06-01 | E-MTAB-11236 | biostudies-arrayexpress