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Metabolic Remodeling during Nitrogen Fixation in Zymomonas

ABSTRACT: This project investigates the proteomic remodeling associated with N2 fixation and NH4+ assimilation in Zymomona mobilis. Previous work has elucidated the metabolic and proteomic response of Zymomona mobilis during steady state N2 fixation. These experiments examine the proteomic profile during the dynamic shift of N2 and NH4+.

INSTRUMENT(S):

ORGANISM(S): Zymomonas Mobilis Subsp. Mobilis Zm4 = Atcc 31821

SUBMITTER: Katherine Overmyer

LAB HEAD: Joshua J Coon

PROVIDER: PXD028526 | Pride | 2021-10-07

REPOSITORIES: Pride

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			Action	DRS
	20200228_EAT_G00007460.raw	Raw
	20200228_EAT_G00007461.raw	Raw
	20200228_EAT_G00007462.raw	Raw
	20200228_EAT_G00007464.raw	Raw
	20200228_EAT_G00007467.raw	Raw

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Project description:Background Zymomonas mobilis ZM4 is a capable ethanologenic bacterium with high ethanol productivity and ethanol tolerance. Previous studies indicated that several stress-related proteins and changes in the ZM4 membrane lipid composition may contribute to ethanol tolerance. However, the molecular mechanisms of its ethanol stress response have not been elucidated fully. Methodology/Principal Findings In this study, ethanol stress responses were investigated using systems biology approaches. Medium supplementation with an initial 47 g/L (6% v/v) ethanol reduced Z. mobilis ZM4 glucose consumption, growth rate and ethanol productivity compared to that of untreated controls. A proteomic analysis of early exponential growth identified about one thousand proteins, or approximately 55% of the predicted ZM4 proteome. Proteins related to metabolism and stress response such as chaperones and key regulators were more abundant in the early ethanol stress condition. Transcriptomic studies indicated that the response of ZM4 to ethanol is dynamic, complex and involves many genes from all the different functional categories. Most down-regulated genes were related to translation and ribosome biogenesis, while the ethanol-upregulated genes were mostly related to cellular processes and metabolism. Transcriptomic data were used to update Z. mobilis ZM4 operon models. Furthermore, correlations among the transcriptomic, proteomic and metabolic data were examined. Among significantly expressed genes or proteins, we observe higher correlation coefficients when fold-change values are higher. Conclusions Our study has provided insights into the responses of Z. mobilis to ethanol stress through an integrated “omics” approach for the first time. This systems biology study elucidated key Z. mobilis ZM4 metabolites, genes and proteins that form the foundation of its distinctive physiology and its multifaceted response to ethanol stress. A sixteen array study using total RNA recovered from wild-type cultures of Zymomonas mobilis subsp mobilis ZM4 at different time points of 6, 10, 13.5, and 26h post-inoculation with 6% (v/v) treatment compred to that of control without ethanol supplementation. Two biological replicates for treatment and control condition.

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Metabolic Remodeling during Nitrogen Fixation in Zymomonas

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