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Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development

ABSTRACT: To characterize vitreous humor (VH) exosomes and to explore their role in the development of proliferative vitreoretinopathy (PVR) using mass-spectrometry based proteome profiling.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Vitreous Chamber Of The Eye

DISEASE(S): Retinal Detachment,Proliferative Vitreoretinopathy,Macular Holes

SUBMITTER: Virginie Sjoelund

LAB HEAD: Lee Sun Young

PROVIDER: PXD028539 | Pride | 2022-06-09

REPOSITORIES: Pride

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Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development.

Nair Gopa Kumar Gopinadhan GKG Pollalis Dimitrios D Wren Jonathan D JD Georgescu Constantin C Sjoelund Virginie V Lee Sun Young SY

Journal of clinical medicine 20220511 10

Purpose: To characterize vitreous humor (VH) exosomes and to explore their role in the development of proliferative vitreoretinopathy (PVR) using mass spectrometry-based proteome profiling. Methods: Exosomes were isolated from undiluted VH from patients with retinal detachment (RD) with various stages of PVR (n = 9), macular hole (MH; n = 5), or epiretinal membrane (ERM; n = 5) using differential ultracentrifugation. The exosomal size, morphology, and exosome m ...[more]

PMID: 35628842

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