Glucose dissociates DDX21 dimers to regulate mRNA splicing and promote epidermal differentiation

ABSTRACT: We discovered that glucose directly bind to DDX21, induced the conformation change of the proteins and inhibit its helicase activity. Glucose also inhibits the dimerization of DDX21, re-localize DDX21 from nucleolus to nucleoplasm and reassemble DDX21 to RNA splicing complex. DDX21 is essential for epidermal differentiation while with normal glucose condition, DDX21 was re-localized from nucleolus to nucleoplasm, bind to splicing factors and mRNA introns more to regulate splicing of key differentiation factors and promote epidermal differentiation.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Keratinocyte

SUBMITTER: Yen-Yu Yang  

LAB HEAD: Yinsheng Wang

PROVIDER: PXD028561 | Pride | 2023-01-05

REPOSITORIES: Pride