Proteomics

Dataset Information

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LC-MS/MS Analysis of Respiratory Viruses


ABSTRACT: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and other respiratory viruses -Coronavirus OC43, Coronavirus 229E, Influenza A/H1N1, Influenza A/H3N2, Influenza B, Respiratory Syncytial Virus RSV A and RSV B - were analysed by bottom-up proteomics of viral cultures. High coverage of viral proteins was acheived after culturing in serum-free conditions when compared to cultures grown using standard conditions including 2% fetal bovine serum.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Human Coronavirus Oc43 Homo Sapiens (human) Influenza A Virus (a/swine/qc/440-a/2009(h3n2)) Bos Taurus (bovine) Severe Acute Respiratory Syndrome Coronavirus 2 Human Coronavirus 229e Human Respiratory Syncytial Virus A Chlorocebus Sabaeus Influenza B Virus (b/victoria/500/2014) Human Respiratory Syncytial Virus B Influenza A Virus (a/swine/qc/705-a/2009(h1n1))

TISSUE(S): Epithelial Cell

DISEASE(S): Severe Acute Respiratory Syndrome,Influenza

SUBMITTER: Christopher Hodgkins  

LAB HEAD: William D. Rawlinson

PROVIDER: PXD028562 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

Simultaneous monitoring of eight human respiratory viruses including SARS-CoV-2 using liquid chromatography-tandem mass spectrometry.

Hodgkins Christopher C   Buckton Laura K LK   Walker Gregory J GJ   Crossett Ben B   Cordwell Stuart J SJ   Horvath Andrea R AR   Rawlinson William D WD  

Scientific reports 20220804 1


Diagnosis of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection has primarily been achieved using reverse transcriptase polymerase chain reaction (RT-PCR) for acute infection, and serology for prior infection. Assay with RT-PCR provides data on presence or absence of viral RNA, with no information on virus replication competence, infectivity, or virus characterisation. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is typically not used in clinical virology, despit  ...[more]

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