Proteomics

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Human whole blood processed with VAMS device

ABSTRACT: This project tested the use of a volumemetric absorptive microsampling device as a substrate to prepare whole blood for proteomic analysis by LC-MS. We demonstrated that VAMS are a useful substrate to trap proteins from blood, wash them to reduce highly abundant protein species which enabled single shot LC-MS. This led to the detection of more than 1600 proteins in a single run. We further tested different washing reagents and compared VAMS against paper DBS. Finally, we demonstrated an application of using VAMS to prepare archival, frozen whole blood cell pellets from non-small cell lung cancer patients for biomarker studies.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood

SUBMITTER: Mark Molloy  

LAB HEAD: Mark P Molloy

PROVIDER: PXD028605 | Pride | 2022-02-21

REPOSITORIES: Pride

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Publications

Proteomic Analysis of Whole Blood Using Volumetric Absorptive Microsampling for Precision Medicine Biomarker Studies.

Molloy Mark P MP   Hill Cameron C   O'Rourke Matthew B MB   Chandra Jason J   Steffen Pascal P   McKay Matthew J MJ   Pascovici Dana D   Herbert Ben R BR  

Journal of proteome research 20220215 4

Microsampling of patient blood promises several benefits over conventional phlebotomy practices to facilitate precision medicine studies. These include at-home patient blood collection, supporting telehealth monitoring, minimal postcollection processing, and compatibility with nonrefrigerated transport and storage. However, for proteomic biomarker studies, mass spectrometry of whole blood has generally been avoided in favor of using plasma or serum obtained from venepuncture. We evaluated the us  ...[more]

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