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EIF5A hypusinaion in hepatic cells

ABSTRACT: determin the impact fatty acids and spermidine on EIF5A hysusinaion, and examined the role of EIF5A hysusination in hepatocytes

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hepatocyte, Cell Culture

SUBMITTER: Jin Zhou

LAB HEAD: Jin Zhou

PROVIDER: PXD028714 | Pride | 2022-09-21

REPOSITORIES: Pride

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Spermidine-mediated hypusination of translation factor EIF5A improves mitochondrial fatty acid oxidation and prevents non-alcoholic steatohepatitis progression.

Zhou Jin J Pang Jeremy J Tripathi Madhulika M Ho Jia Pei JP Widjaja Anissa Anindya AA Shekeran Shamini Guna SG Cook Stuart Alexander SA Suzuki Ayako A Diehl Anna Mae AM Petretto Enrico E Singh Brijesh Kumar BK Yen Paul Michael PM

Nature communications 20220903 1

Spermidine is a natural polyamine that has health benefits and extends life span in several species. Deoxyhypusine synthase (DHPS) and deoxyhypusine hydroxylase (DOHH) are key enzymes that utilize spermidine to catalyze the post-translational hypusination of the translation factor EIF5A (EIF5A<sup>H</sup>). Here, we have found that hepatic DOHH mRNA expression is decreased in patients and mice with non-alcoholic steatohepatitis (NASH), and hepatic cells treated with fatty acids. The mouse and ce ...[more]

PMID: 36057633

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