P300 acetylates Ub and linear Ub dimers

ABSTRACT: In this project we present the identification of in vitro acetylation of Ub by the acetyltransferase p300. Free monomeric Ub or M1-linked linear Ub dimers (diUb) were incubated in vitro with p300 in presence or absence of acetyl-Coenzyme A (AcCoA). For identification of the acetylation status of the respective Ub variants parallel reaction monitoring (PRM), a targeted-MS approach, was used.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Simon Kienle  

LAB HEAD: Prof. Dr. Martin Scheffner

PROVIDER: PXD028813 | Pride | 2022-10-14

REPOSITORIES: Pride