I.e. mouse ovary palmitoylation protein LC-MSMS

ABSTRACT: To identify a role for protein palmitoylation in ovarian of DHEA-induced PCOS mice model. First, we used DHEA to induce a PCOS mice model，hematoxylin-eosin (H&E) staining sections form the ovaries of the Control and DHEA groups.The mice oral gavage with DHEA disrupted ovarian morphology,the estrous cycle, and hormone profilein DHEA-induced mouse. The three pairs of ovary for each tube are mixed together as a group were lysed and divided into 2 fractions. The -HAM condition served as a negative control and the +HAM sample positive control.In the +HAM sample, the palmitate residue was cleaved off and exchanged with biotin. After the ABE reaction was completed, streptavidin beads were used to enrich for biotinylated proteins. Proteins enriched from +HAM and −HAM conditions were identified using mass spectrometry (MS).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Ovary

DISEASE(S): Polycystic Ovary Syndrome

SUBMITTER: Tang Ting  

LAB HEAD: Bing Yao

PROVIDER: PXD028835 | Pride | 2023-03-11

REPOSITORIES: Pride